Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. To learn more about the device, please watch this short video. Molecular Devices Software. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Then, the cell area was calculated for each. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Examples: fiPress carefully to engage,fl fiDo not press down. Ease of use. ValitaTiter Plus settingsThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Eliminate time-dependent substrate addition steps. 11. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 08, 0. Transfected cells were imaged on the SpectraMax MiniMax 300 Imaging Cytometer. Published Dec 16, 2020 | Updated Dec 16, 2020. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). Microplate optimization should be performed the first time the assay is run, and subsequently for . The protocols are also available on the SoftMax Pro Software protocol sharing. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The dual auto injector module. 06, 0. Get a front row view of your cells. Art-science interactive light show/animation. 58% for the detection module and 2. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. techsupport. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. SpectraMax i3x Multi-Mode Detection Platform User Guide. Prime function 20 amol. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. D 1 1. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. 313 reference gain check fail. 313 reference gain check fail. 306 plate air cal fail,low light. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. *On the SpectraMax i3x reader only. See SpectraMax® Microplate Reader User Manual Download Page. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. Figure 1. Nuclear Receptor. 2. 0´ 104 1. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. i3x. 1. 2. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The calculated LOQ values were 0. Get a front row view of your cells. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. Molecular Devicesの全商品を見る. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. no. Bioz Stars score: 98/100, based on 1 PubMed citations. Explore a wealth of applications with the future-ready SpectraMax i3x. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Increased fluorescence performance. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. 12911151) 0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Molecular Devices Corporation. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. pathways and protein activation and expression in one system. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Downloads. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. ZERO BIAS - scores, article reviews, protocol conditions and more. SeeSpectraMax MiniMaxImagingCytometeronpage85. Expand your lab’s capabilities to include flash applications such as dual luciferase and. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. Overview of SpectraMax MiniMax 300 Imaging Cytometer. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). We would like to show you a description here but the site won’t allow us. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. 발광 (Luminescence) 리더기. Automated for ease of use. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 3: The cuvette chamber. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Fluorescence Reader. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Contact Us Phone: +1. These concentrations are then used to perform western blots. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Detection of pro-inflammatory factors. Data Analysis. 312 gain calibration failed. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. *On the SpectraMax i3x reader only. With optimized reagents, option to operate the readers. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. Register to download your free eBook today. Patented user-exchangeable cartridge design expands the system's detection capability making it. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Description 1. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The plate was shaken and incubated in the dark for 10 minutes. Recommended settings for SpectraMax iD5 and i3x readers. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. It allows you to explore cellular pathways and protein activation and expression in one system. When the user applies a set temperature of 37°C, the top is heated to 37. 4. Multimode Reader Comparison Chart. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. AquaMax Microplate Washer. 3. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. iD5. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Introduction. Read height should be optimized for . Example: [Drawer]. QBT Fatty Acid Transporter Assay. Check software compatibility with Windows 10 and locate your software installer. Prismatic colour and motion for everyone. 1) Select the correct plate and the wells that you want to measure. Spectramax I3x, supplied by Danaher Inc, used in various techniques. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Learn more about its features, specifications, and applications in this user guide. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. SpectraMax i3x. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Multimode Reader Comparison Chart. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. CRITICAL STEP Avoid air bubbles; reverse pipetting the luciferase reagent into the plate is a good way to achieve this. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. 蛍光 発光 ELISA. The SpectraMax ® i3x reader provides the. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Pick the best speed, sensitivity, and price for your needs. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Unlock your i3/i3x instrument after shipment. 99. Image analysis provides accurate data. Find regional support. Multimode Reader Comparison Chart. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. Bioz Stars score: 98/100, based on 1 PubMed citations. See SpectraMax® Microplate Reader User Manual Download Page. The calculated LOQ values were 0. Note: Additional readers with similar performance are listed. Category 2 and 3 microplate readers use gradient heating as well. Supplier: Molecular Devices. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. 0´ 104 4. SpectraMax ® iD3 and iD5 Multi-Mode . 2 (Molecular Devices, San Jose, CA). SpectraMaxi3Multi. Synthesis of ZM-1NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 4. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. Multimode Readers Comparison Chart. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Spectranet customer portalSpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer by Molecular Devices® Manufacturer Molecular Devices® | Model: i3x | Available Worldwide 4. Introduction. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Prime function 20 amol. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Find regional support. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 5 µL anti-cAMP-Cryptate. The SoftMax Pro Software quickly. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Thetypeof luciferase(Renilla. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. 200-800 nm using Spectra Max i3X microplate reader. ATP (“flash” luminescence using . The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. 6 in. Italic and boldface type are used for emphasis. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. i3x. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Figure 4. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The SoftMax Pro Software provides integrated instrument control. SpectraMax i3x Multi-Mode Detection Platform. 13. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Increased fluorescence performance. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Sunnyvale, CA, USA) with excitation at 475 nm. 2. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. ATP (“flash” luminescence using . This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. It also integrates with the. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. com. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Next,. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Molecular Devices are known for both the reliability and the wide uses of their products. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. 6 in. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Microplate reader assays requiring infrared (IR) or red-shifted. 0´ 104 6. It allows you to explore cellular pathways and protein activation and expression in one system. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. ) deep, preferably with access on all sides. The corrected absorbance is within 5% of the value. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. More Product Information. ) wide and 65 cm (25. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength and superior sensitivity across the full. . ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . Register to download your free eBook today. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. 1. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. Example: [Drawer]. eu@moldev. Also refer to this Multimode Reader Comparison Chart. Replicate values were averaged and imported into Design-Expert 8. Simplified workflow: Add cellular imaging assays in a small footprint. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SpectraMax i3x from Molecular Devices is a multi-mode plate reader that can measure spectral-based absorbence and luminescence. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Published Dec 16, 2020 | Updated Dec 16, 2020. china@moldev. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Home > Search Results > Danaher Inc > spectramax i3x. Apoptosis was measured by the Annexin V-FITC/PI Apoptosis Detection Kit. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. 304 signal level saturation. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Find regional support. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 3) At the bottom of the strip, click on "Show. Microplate Reader. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. Bound crystal violet was dissolved with 10% acetic acid and the optical density (OD) at 570 nm was determined using a Spectra Max i3X spectrophotometer (Molecular Devices). Increased fluorescence performance. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. pathways and protein activation and expression in one system. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . The chamber. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). 価格、見積もり、お近くの販売店を. SoftMax Pro GxP Compliance Software. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. each new lot of plates, to ensure that the center of each well is read. In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. 315 can't find zero order. 2 (Molecular Devices, San Jose, CA, USA). Find regional support. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Enzyme Activation Analysis;. . Time-. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 4 / 5. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. 3) At the bottom of the strip, click on "Show. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Patented user-exchangeable cartridge design expands the system's detection capability making it. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Promega. Explore a wealth of applications with the future-ready SpectraMax i3x. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. infoboxeu@moldev. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Controls containing all reaction components with either DI . Description 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The Molecular Devices SpectraMax i3x is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. The treatments were. Multimode Readers Comparison Chart. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The standard solutions (0. Native or oxidized AN (40 μM) and FN (0. With a preconfigured protocol to simplify data acquisition and analysis, SoftMax Pro Software completes this total solution for fast, sensitive, and reliable dual luciferase reporter assay results. Other services inlcude but are not limited to anti-drug activity (ADA), PK/PD assays as well as antibody screenings. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. *On the SpectraMax i3x reader only. To detect physical stability, Pdots were treated with water and incubated with plants. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. Figure 3. 0.